Monolayer Cell Cultures from Marine Fishes

Propagation of goat pox virus on monolayer cell cultures

Monolayer Cultures Derived from Neonatal Hamster Pancreas

Cells derived by trypsinization of neonatal golden hamster pancreas were cultured in modified Eagle's medium for 120 h in the presence of glucose (0.8 mg/ml) and for an additional 48 h in medium containing glucose (0.8 or 3.1 mg/ml) or tolbutamide (1,000 microg/ml) plus glucose (0.8 mg/ml). At day 7, cultures were stained differentially for light microscopy or examined by electron microscopy. I...

Propagation of goat pox virus on monolayer cell cultures.

Although the majority of different members of poxviruses have been carefully studied, !ittle is known about goat pox and no detailed report on the disease and its causative agent is, so far, available. The cultivation of goat pox virus (GPV) in cell cultures reported in the present paper makes available the most reliable and economic source for large s~ale produc~ion of vaccine to control the d...

Rapid screening of lipid metabolism in monolayer cell cultures.

Monolayer cell cultures grown on coverslips in the presence of radioactive lipid precursors were embedded in silica gel layers for extraction and resolution of the labelled products directly by thin-layer chromatography. The method permits rapid screening of lipid metabolism in tissue cultures with a small number of cells.

Freeze-fracture of monolayer cultures

This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze-fracturing.